Our second, improved generation of our technology has been published in the prestigious international journal JACS (Impact factor of 13).
Engineered PQQ-glucose dehydrogenase as a universal biosensor platform

Zhong Guo, Lindy Murphy, Viktor Stein, Wayne Johnston, Siro Perez-Alcala, and Kirill Alexandrov

J. Am. Chem. Soc., Just Accepted Manuscript
DOI: 10.1021/jacs.6b06342
Publication Date (Web): July 27, 2016
Copyright © 2016 American Chemical Society

Biosensors with direct electron output hold the promise of nearly seamless integration with portable electronic devices. However, so far they are based on naturally occurring en-zymes that significantly limits the spectrum of detectable analytes. Here we present a novel biosensor architecture based on analyte driven intermolecular recombination and activity reconstitution of reengineered component of glu-cometers; PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted to the detection of immunosuppressant drugs, alpha-amylase protein or protease activity of thrombin and Factor Xa. The biosensors could be stored in dried form without the appreciable loss of activity and their activity could be directly monitored by chronoamperometry enabling construction of the dis-posable sensory electrodes. We expect that this architecture could be expanded to the detection of other biochemical activities, posttranslational modifications, nucleic acids and inorganic molecules.